An oligoarray for the detection of human papillomavirus type 16 variants.
Identifieur interne : 005442 ( Main/Exploration ); précédent : 005441; suivant : 005443An oligoarray for the detection of human papillomavirus type 16 variants.
Auteurs : P. Mendoza-Lorenzo [Mexique] ; R. Maldonado ; R. Pacheco ; A. Méndez ; P. Pi A-Sánchez ; A. Rangel ; G. Vazquez-Ortíz ; M. SalcedoSource :
- International journal of gynecological cancer : official journal of the International Gynecological Cancer Society [ 1048-891X ]
Descripteurs français
- KwdFr :
- Analyse de mutations d'ADN (), Femelle, Humains, Mutation, Papillomavirus humain de type 16 (génétique), Papillomavirus humain de type 16 (isolement et purification), Protéines de répression (génétique), Protéines des oncogènes viraux (génétique), Réaction de polymérisation en chaîne, Sondes d'ADN (), Séquençage par oligonucléotides en batterie ().
- MESH :
- génétique : Papillomavirus humain de type 16, Protéines de répression, Protéines des oncogènes viraux.
- isolement et purification : Papillomavirus humain de type 16.
- Analyse de mutations d'ADN, Femelle, Humains, Mutation, Réaction de polymérisation en chaîne, Sondes d'ADN, Séquençage par oligonucléotides en batterie.
English descriptors
- KwdEn :
- DNA Mutational Analysis (methods), DNA Probes (chemistry), Female, Human papillomavirus 16 (genetics), Human papillomavirus 16 (isolation & purification), Humans, Mutation, Oligonucleotide Array Sequence Analysis (methods), Oncogene Proteins, Viral (genetics), Polymerase Chain Reaction, Repressor Proteins (genetics).
- MESH :
- chemical , chemistry : DNA Probes.
- genetics : Human papillomavirus 16, Oncogene Proteins, Viral, Repressor Proteins.
- isolation & purification : Human papillomavirus 16.
- methods : DNA Mutational Analysis, Oligonucleotide Array Sequence Analysis.
- Female, Humans, Mutation, Polymerase Chain Reaction.
Abstract
On the basis of human papillomavirus (HPV) E6 gene mutations, there are more than five variants of HPV 16. We applied a sensitive and specific stacking hybridization assay using an oligoarray for the detection of Asian-American (AA) and European (E) (E350G) HPV 16 variants. A simple glass slide was coated with capture probes consisting of short oligonucleotide DNA sequences (7-9 mers) specific for AA and E variants. Two different regions of the E6 HPV 16 gene were amplified with a set of two primers, which were used as target DNA. These targets were preannealed with auxiliary labeled oligonucleotides and hybridized to the oligoarray in the presence of specific and complementary capture probes. Our designed array based on shorter capture probes successfully discriminated between HPV 16 AA and E variants. The present DNA oligoarray system could be useful as a reliable technique for HPV 16 detection and does not require specialized equipment; nevertheless, further intra- and interlaboratory studies are needed.
DOI: 10.1111/j.1525-1438.2007.00832.x
PubMed: 17877642
Affiliations:
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Mendoza-Lorenzo</name><affiliation wicri:level="1"><nlm:affiliation>Oncogenomics Laboratory, Oncology Diseases Research Unit, National Medical Center Siglo XXI-IMSS, Av. Mexico D.F., Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Oncogenomics Laboratory, Oncology Diseases Research Unit, National Medical Center Siglo XXI-IMSS, Av. Mexico D.F.</wicri:regionArea><wicri:noRegion>Av. Mexico D.F.</wicri:noRegion></affiliation></author><author><name sortKey="Maldonado, R" sort="Maldonado, R" uniqKey="Maldonado R" first="R" last="Maldonado">R. Maldonado</name></author><author><name sortKey="Pacheco, R" sort="Pacheco, R" uniqKey="Pacheco R" first="R" last="Pacheco">R. Pacheco</name></author><author><name sortKey="Mendez, A" sort="Mendez, A" uniqKey="Mendez A" first="A" last="Méndez">A. Méndez</name></author><author><name sortKey="Pi A Sanchez, P" sort="Pi A Sanchez, P" uniqKey="Pi A Sanchez P" first="P" last="Pi A-Sánchez">P. Pi A-Sánchez</name></author><author><name sortKey="Rangel, A" sort="Rangel, A" uniqKey="Rangel A" first="A" last="Rangel">A. Rangel</name></author><author><name sortKey="Vazquez Ortiz, G" sort="Vazquez Ortiz, G" uniqKey="Vazquez Ortiz G" first="G" last="Vazquez-Ortíz">G. Vazquez-Ortíz</name></author><author><name sortKey="Salcedo, M" sort="Salcedo, M" uniqKey="Salcedo M" first="M" last="Salcedo">M. 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The present DNA oligoarray system could be useful as a reliable technique for HPV 16 detection and does not require specialized equipment; nevertheless, further intra- and interlaboratory studies are needed.</div></front></TEI><affiliations><list><country><li>Mexique</li></country></list><tree><noCountry><name sortKey="Maldonado, R" sort="Maldonado, R" uniqKey="Maldonado R" first="R" last="Maldonado">R. Maldonado</name><name sortKey="Mendez, A" sort="Mendez, A" uniqKey="Mendez A" first="A" last="Méndez">A. Méndez</name><name sortKey="Pacheco, R" sort="Pacheco, R" uniqKey="Pacheco R" first="R" last="Pacheco">R. Pacheco</name><name sortKey="Pi A Sanchez, P" sort="Pi A Sanchez, P" uniqKey="Pi A Sanchez P" first="P" last="Pi A-Sánchez">P. Pi A-Sánchez</name><name sortKey="Rangel, A" sort="Rangel, A" uniqKey="Rangel A" first="A" last="Rangel">A. Rangel</name><name sortKey="Salcedo, M" sort="Salcedo, M" uniqKey="Salcedo M" first="M" last="Salcedo">M. Salcedo</name><name sortKey="Vazquez Ortiz, G" sort="Vazquez Ortiz, G" uniqKey="Vazquez Ortiz G" first="G" last="Vazquez-Ortíz">G. Vazquez-Ortíz</name></noCountry><country name="Mexique"><noRegion><name sortKey="Mendoza Lorenzo, P" sort="Mendoza Lorenzo, P" uniqKey="Mendoza Lorenzo P" first="P" last="Mendoza-Lorenzo">P. Mendoza-Lorenzo</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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